. 2017 Oct 20;5:e3945. doi: 10.7717/peerj.3945

Table 3. M. buryatense 5GB1C mutant characteristics: bold-face, mutants with phenotypes; italics, no null mutants obtained.

Gene(s) deleted	Enzyme	Locus tag(s)	Phenotypes	Category
ack	acetate kinase	METBUDRAFT_1552	none	fermentation product: acetate
acs	acetylCoA synthase	METBUDRAFT_1565	none	fermentation product: acetate
ack/acs	acetate kinase/acetylCoA synthase (double)	METBUDRAFT_1552/ METBUDRAFT_1565	no null mutants	fermentation product: acetate
pta	phosphotransacetylase	METBUDRAFT_1957	none	fermentation product: acetate
xfp1	phosphoketolase	METBUDRAFT_1551	none	fermentation product: acetate
xfp2	phosphoketolase	METBUDRAFT_0185	none	fermentation product: acetate
xfp1/xfp2	phosphoketolase (double)	METBUDRAFT_1551/ METBUDRAFT_0185	none	fermentation product: acetate
ldh	lactate dehydrogenase	METBUDRAFT_3726	none	fermentation product: lactate
mdh	malate dehydrogenase	METBUDRAFT_2208	none	fermentation product: succinate
malic_enzyme	malic enzyme	METBUDRAFT_2185	none	fermentation product: succinate
hox	hydrogenase	METBUDRAFT_1387-1390	none	fermentation product: H₂
ndh1	NADH dehydrogenase	METBUDRAFT_1319	none	NADH utilization
*ndh2*	NADH dehydrogenase	METBUDRAFT_2827	formate up; acetate and succinate down	NADH utilization
*ndh1/ndh2*	NADH dehydrogenase (double)	METBUDRAFT_1319/ METBUDRAFT_2827	formate up, acetate and succinate down	NADH utilization
petABC	bc1 complex	METBUDRAFT_2502-2504	none	NADH utilization
*ctaCD*	cytochrome oxidase aa3-type	METBUDRAFT_0311-0312	formate up; acetate and succinate down; growth rate down	NADH utilization
cba	cytochrome oxidase cba-type	METBUDRAFT_1311-1313	none	NADH utilization
gnd1	6-phosphogluconate dehydrogenase (NADP)	METBUDRAFT_3313	none	NADH generation
gnd2	6-phosphogluconate dehydrogenase (NAD)	METBUDRAFT_3982	none	NADH generation
gnd1/gnd2	6PGDH (double)	METBUDRAFT_3313/ METBUDRAFT_3982	none	NADH generation
mtd1	methylene H4MPT dehydrogenase	METBUDRAFT_1893	no null mutants	NADH generation
mtd2	methylene H4MPT dehydrogenase	METBUDRAFT_1894	no null mutants	NADH generation
fdh1ab	formate dehydrogenase (tungsten)	METBUDRAFT_0831-0832	none	NADH generation
*fdh2*	formate dehydrogenase (molybdenum)	METBUDRAFT_2831	formate up; acetate and succinate down	NADH generation
*fdh1/fdh2*	formate dehydrogenase double	METBUDRAFT_0831-0832/METBUDRAFT_2831	formate up; acetate and succinate down; growth rate down	NADH generation
bhr	bacteriohemerythrin	METBUDRAFT_0310	none	O₂ starvation