Table 4. Flux balance analysis results.

Experiment ID	FM86	FM87	FM88	FM90
Strain	5GB1 WT	5GB1 WT	5GB1 ΔCyt aa3	5GB1 ΔCyt aa3
Experimental growth rate (h−1)	0.036	0.035	0.034	0.035
Growth rate from simulation case1 (specific methane uptake rate constraint)	0.04	0.043	NA	NA
Growth rate from simulation case2 (adding O2 uptake rate constraint to case 1)	0.034	0.036	NA	NA
Growth rate from simulation case3 (adding secreted products constraints to case 2)	0.033	0.034	NA	NA
Growth rate from simulation case4 (ΔCyt aa3 with methane, O2 uptake rate and secreted products constraint)	NA	NA	0.043	0.048