Fig. 2.3.

Regulated translation of the mRNAs encoding proteins of the BMP pathway. (a) The isolation of polyribosomes from Xenopus oocytes, eggs, and embryos (Sheets et al. 2010). At the right, total (unfractionated) RNA from eggs, non-polyribosomal RNA from eggs, and polyribosomal RNA from eggs were analyzed with blot hybridization using probes to the cyclin B1 and cytoskeletal actin mRNAs. (b) Polyribosome and non-polyribosome fractions were prepared from Xenopus laevis oocytes, eggs, and stage 7 blastula embryos (Fritz and Sheets 2001). Total RNAs isolated from the fractions were analyzed by blot hybridization. Filters were hybridized with probes to detect Xenopus mRNAs encoding the Smad1 transcription factor, the BMP7 ligand, BMPR1a receptor, and cytoskeletal actin proteins. A representative RNA blot analyzing each mRNA is shown. Recreated from Fritz, B.R. and M.D. Sheets Developmental Biology, 2001. 236(1): p. 230–243, with permission from Elsevier