Fig. 5.
Effect of hIL-1RA on the microglial pro-inflammatory response to LPS ex vivo. Four days post-ICM injection of vehicle (0.9%) or hIL-1RA (100 μg), hippocampal microglia were isolated, exposed to LPS ex vivo for 2 h and the pro-inflammatory cytokine response measured. Open bars indicate vehicle treatment and closed bars indicate hIL-1RA treatment in vivo. Insets: For IL-1 and IL-6, area under the LPS concentration curve (AUC) was computed for vehicle and hIL-1RA treatment groups and means compared (*p<.05). Data are presented as the mean±SEM.