Table 1.
Primer Sequences.
| Gene | Primer sequence 5′ → 3′ | Function |
|---|---|---|
| β-Actin | F: TTCCTTCCTGGGTATGGAAT R: GAGGAGCAATGATCTTGATC |
Cytoskeletal protein(housekeeping gene) |
| CD163 | F: GTAGTAGTCATTCAACCCTCAC R: CGGCTTACAGTTTCCTCAAG |
Macrophage specific antigen |
| CD200R | F: TAGAGGGGGTGACCAATTAT R: TACATTTTCTGCAGCCACTG |
Microglia/macrophage antigen |
| GFAP | F: AGATCCGAGAAACCAGCCTG R: CCTTAATGACCTCGCCATCC |
Astrocyte antigen |
| IL-1β | F: CCTTGTGCAAGTGTCTGAAG R: GGGCTTGGAAGCAATCCTTA |
Pro-inflammatory cytokine |
| IL-1R1 | F: TAGATGACAGCAAGAGGGA R: ACTTCCAGTAGACAAGGTC |
Receptor mediating IL-1β pro-inflammatory signaling |
| IL-6 | F: AGAAAAGAGTTGTGCAATGGCA R: GGCAAATTTCCTGGTTATATCC |
Pro-inflammatory cytokine |
| Iba-1 | F: GGCAATGGAGATATCGATAT R: AGAATCATTCTCAAGATGGC |
Microglia/macrophage antigen |
| MHCII | F: AGCACTGGGAGTTTGAAGAG R: AAGCCATCACCTCCTGGTAT |
Microglia/macrophage antigen |
| NFκBIα | F: CACCAACTACAACGGCCACA R: GCTCCTGAGCGTTGACATCA |
Induced by NFκB to inhibit NFκB function |
| TNFα | F: CAAGGAGGAGAAGTTCCCA R: TTGGTGGTTTGCTACGACG |
Pro-inflammatory cytokine |
| TLR2 | F: TGGAGGTCTCCAGGTCAAATC R: ACAGAGATGCCTGGGCAGAAT |
Pattern recognition receptor for motifs of gram-positive bacteria |
| TLR4 | F: TCCCTGCATAGAGGTACTTC R: CACACCTGGATAAATCCAGC |
Pattern recognition receptor for motifs of gram-negative bacteria |