. Author manuscript; available in PMC: 2017 Oct 23.

Published in final edited form as: Eur J Med Chem. 2016 Jul 22;123:317–331. doi: 10.1016/j.ejmech.2016.07.045

Table 2.

The inhibitory activities (IC₅₀) of ursolic acid (UA 1), derivative 2, and the most potent derivatives (15–17) in spheroid model of the H460 and H322 lung cancer cell lines.

Cell line^(a)/ IC₅₀, µM
Compound	H460		H322

	Monolayer	Spheroid	Monolayer	Spheroid
UA 1	14.8±0.6	30.8±2.8	15.3±2.8	18.2±10.5
2	19.3±2.6	19.5±10.4	14.3±2.7	ND
15	4.5±0.4	3.0±1.2	6.8±1.5	12.1±1.7
16	5.3±0.3	5.7±0.3	7.3±1.0	11.6±7.4
17	2.6±0.9	5.5±0.8	3.3±0.9	10.0±1.2

^(a)

Lung cancer cell lines were treated with various concentrations of test compounds for 72h or 96h for a 2D (monolayer) or 3D model (spheroid), respectively. The inhibitory activities were determined using CellTiter-Blue® or CellTiter-Glo® for the 2D or 3D model, respectively. IC₅₀ was calculated from the results, and data are presented as means ± SD of three independent experiments.