Table 1.
Primer description and sequence.
| Gene | Primer sequence 5′ → 3′ | Function |
|---|---|---|
| β-Actin | F: TTCCTTCCTGGGTATGGAAT | Cytoskeletal protein (Housekeeping gene) |
| R: GAGGAGCAATGATCTTGATC | ||
| CD163 | F: GTAGTAGTCATTCAACCCTCAC | Macrophage antigen not expressed by microglia |
| R: CGGCTTACAGTTTCCTCAAG | ||
| GFAP | F: AGATCCGAGAAACCAGCCTG | Astrocyte antigen |
| R: CCTTAATGACCTCGCCATCC | ||
| IL-1β | F: CCTTGTGCAAGTGTCTGAAG | Pro-inflammatory cytokine |
| R: GGGCTTGGAAGCAATCCTTA | ||
| IL-6 | F: AGAAAAGAGTTGTGCAATGGCA | Pro-inflammatory cytokine |
| R: GGCAAATTTCCTGGTTATATCC | ||
| Iba-1 | F: GGCAATGGAGATATCGATAT | Microglia/macrophage antigen |
| R: AGAATCATTCTCAAGATGGC | ||
| MHCII | F: AGCACTGGGAGTTTGAAGAG | Microglia/Macrophage antigen |
| R: AAGCCATCACCTCCTGGTAT | ||
| NF-κBIα | F: CACCAACTACAACGGCCACA | Induced by NFκB to inhibit NFκB function |
| R: GCTCCTGAGCGTTGACATCA | ||
| TNFα | F: CAAGGAGGAGAAGTTCCCA | Pro-inflammatory cytokine |
| R: TTGGTGGTTTGCTACGACG |
Abbreviations: GFAP, glial fibrillary acidic protein; IL, interleukin; Iba-1, ionized calcium-binding adaptor molecule-1; MHCII, major histocompatibility complex II; NF-κBIα, nuclear factor kappa light chain enhancer of activated B cells inhibitor alpha; TNFα, tumor necrosis factor-α.