Fig A3.

MAGE-A3 (melanoma-associated antigen-A3) T-cell receptor (TCR)–transduced CD4⁺ T cells did not convert to regulatory T cells after cell infusion. (A) Intracellular staining for FOXP3 antibody was performed for peripheral blood lymphocyte samples before (top) and approximately 1 month after (bottom) T-cell therapy. (B) Percentage of CD4⁺mTCRβ⁺FOXP3⁺ cells in lymphocyte populations before (top) and 1 month after (bottom) cell infusion. (C) Percentage of CD4⁺mTCRβ⁻FOXP3⁺ cells in lymphocyte populations before (top) and 1 month after (bottom) cell infusion. Statistical significance was determined by Mann-Whitney U test.