Table. Michaelis-Menten steady-state kinetic parameters for vancomycin-resistant Enterococcus faecium wild-type and C119D MurA*.
| Enzyme | UNAG (p value) |
PEP (p value) |
||||||
|---|---|---|---|---|---|---|---|---|
| Km, μmol/L | Vmax, μmol/L/min | kcat/min | kcat/Km, μmol/L/min | Km, μmol/L | Vmax, μmol/min | kcat/min | kcat/Km, μmol/L/min | |
| WT MurA | 382.8 ± 79.5 | 13.9 ± 1.6 | 138.7 ± 16.4 | 0.4 | 229.0 ± 87.201 | 29.5 ± 8.4 | 294.5 ± 83.8 | 1.3 |
| CD119D MurA | 803.2 ± 1,780 (0.02) | 11.9 ± 2.2 (NS) | 119.4 ± 22.2 (NS) | 0.2 | 304.6 ± 35.2 (NS) | 28.6 ± 3.2 (NS) | 285.5 ± 32.1 (NS) | 0.9 |
*Values are mean ± SD for >3 independent experiments unless otherwise indicated. Statistical differences between kinetic parameters for vancomycin-resistant enterococci WT and C119D MurA were assessed by using a paired t-test. MurA, UDP-N-acetylglucosamine enolpyruvyl transferase; NS, not significant; PEP, phosphoenolpyruvate; UNAG, UDP-N-acetylglucosamine; WT, wild type.