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Thermal stability of IgE-Fc and Fcε3–4. The unfolding of IgE-Fc and Fcε3–4 as a function of temperature and urea concentration, measured by DSF, are compared. (A) 0 M urea. (B) 1 M urea. (C) 2 M urea. (D) 3 M urea. (E) 4 M urea. Melting temperatures reported in Table 2 were determined from the first derivative plots calculated from these curves.
