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. 2017 Aug 24;66(11):2903–2914. doi: 10.2337/db17-0187

Figure 2.

Figure 2

rs149483638 prevents splicing in vitro. A: This variant is located at a canonical splice acceptor site and is predicted to cause skipping of exon 2 of IGF2 isoform 2. B: 293T cells were transfected with IGF2 minigenes containing the first three exons and two introns of the IGF2 gene, and either allele of the rs149483638 C>T variant (G>A in the reverse strand) and cDNA were analyzed by ddPCR. This analysis revealed no expression of the IGF2 exon 1-2 junction in cells transfected with the minigene containing the T2D-protective rs149483638 A allele. This was in contrast to the high levels of exon 1-2 splicing detected in cells transfected with the G allele. C: One-dimensional plots of the ddPCR droplets plotted in B. No IGF2 transcript was detected in untransfected samples. ACTB was used as an internal control.