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. 2016 Jun 23;8(44):75808–75821. doi: 10.18632/oncotarget.10257

Figure 4. Activation of COX-2/mPGES-1/PGE2 pathway in the kidney following albumin overload was inhibited by NLRP3 deletion.

Figure 4

qRT-PCR analyses of COX-1 A. COX-2 B. mPGES-1 C. mPGES-2 D. and cPGES E. F. Western blots of COX-2 and mPGES-1, enzymes along the PGE2 synthetic pathway, are induced by albumin loading, but this effect is reversed in the NLRP3−/− mice. G–H. Densitometric analyses of COX-2 (G) and mPGES-1(H) confirm the impression of the immunoblotting. I. EIA of urinary PGE2 excretion is similarly stimulated by albumin overloaded mice while reduced to near normal levels in NLRP3−/− mice injected with albumin. The values represent the means±SDs (n=6 in each group). * p<0.01 vs. WT control. # p<0.01 vs. albumin-overloaded WT mice.