Figure 1. Hinokitiol inhibits self-renewal of BCSCs.

(A, B) The (A) AS-B145 and (B) BT-474 human breast cancer cells were treated with various concentrations of hinokitiol for 72 h and the cell viability was determined by WST1. IC₅₀ values were calculated by GraFit software. (C, D) Self-renewal capability of AS-B145 (C) or BT-474 (D) was determined by mammosphere cultivation. 2×10⁴ cells were used for primary mammosphere formation assay and 5×10³ dissociated primary mammosphere cells were used for secondary mammosphere formation assay. Hinokitiol was treated for 7 days. Scale bar: 100 μm. *, p< 0.05; **, p< 0.01. The experiments were repeated at least two times and data from one experiment were presented.