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. 2017 Jun 16;8(44):76085–76098. doi: 10.18632/oncotarget.18543

Figure 4. MO-OH-Nap induces caspase cleavage in a time-dependent manner which is distinct from the pan-HDAC inhibitor SAHA.

Figure 4

(A) U266 cells were incubated for 12-48 hours in the presence or absence of SAHA (1 μM) or MO-OH-Nap (10 μM). Whole cell lysate was obtained and immunoblot analysis of PARP, cleaved caspases-3, -8 and -9 was performed along with β-tubulin as a loading control. The gels are representative of three independent experiments. (B) U266 cells were incubated for 24 hours in the presence or absence of a caspase-8 inhibitor (Z-IETD-FMK, C8i, 50 μM) and/or SAHA (2.5 μM) or MO-OH-Nap (10 μM). Whole cell lysate was obtained and immunoblot analysis of cleaved caspases-8 and -9 was performed along with β-tubulin as a loading control. The gels are representative of three independent experiments.