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. 2017 Jun 28;8(44):76116–76128. doi: 10.18632/oncotarget.18814

Figure 2. The stromal fibroblasts cultured medium enhanced the abilities of migration and invasion of lung cancer cells.

Figure 2

(A) Morphological characters of lung cancer cells cultured with CAF-CM and NF-CM. (B) CAF-CM and NF-CM stimulated the growth of A549 and SK-MES-1 cells. Lung cancer cells were cultured in DMEM or DMEM mixed with NF-CM or CAF-CM (1:1) for 72 h. Cell growth were measured by CCK-8. (C) Wound healing assays were performed to evaluate cell migration ability after 48 h, representative photographs are presented (×40 magnification) (D) Relative adhension rates were presented. (E) Transwell assays were performed to evaluate cell invasion ability after 48h, representative photographs are presented (×200 magnification). (F) Relative invasive cell number was presented. Values represent the mean ± SD of three independent experiments. ***P < 0.001.