Table 1. EC50 of MTX on several human cancer cell lines.

	Cell line	EC50 of MTX
MCF-7	human breast adenocarcinoma	2.58 × 10−8M
MDA-MB 231	human breast adenocarcinoma	5.06 × 10−7M
T-24	human bladder carcinoma	8.07 × 10−8M
PANC-1	human pancreatic carcinoma	2.34 × 10−8M
HT29	human colon adenocarcinoma	6.09 × 10−8M
A375	human melanoma	3.37 × 10−8M
HT-1376	human bladder carcinoma	1.71 × 10−7M
OVCAR-3	human ovarian carcinoma	8.95 × 10−8M