Table 3. Methylation status of individual genes and associations with disease-free survival using Cox proportional hazards model in 100 patients with N0 lymph-node status.

Gene	Methylation status	Overall (%)	Recurrence events		Adjusted HR (95% CI) †
			Positive (N = 30)	Negative (N = 70)
NPFFR1	Yes	83(83.0)	23	60
	No	17(17.0)	7	10	0.831 (0.348-1.981)
NPFFR2	Yes	82(82.0)	23	59
	No	18(18.0)	7	11	0.656 (0.266-1.617)
HCRTR1	Yes	71(71.0)	22	49
	No	29(29.0)	8	21	1.162 (0.469-2.879)
HCRTR2	Yes	80(80.0)	22	58
	No	20(20.0)	8	12	0.465 (0.192-1.131)
NPY1R	Yes	36(36.0)	14	22
	No	64(64.0)	16	48	1.568 (0.736-3.342)
NPY2R	Yes	38(38.0)	16	22
	No	62(62.0)	14	48	2.492 (1.190-5.215)*
NPY4R	Yes	37(37.0)	12	25
	No	63(63.0)	18	45	0.926 (0.423-2.030)
NPY5R	Yes	34(34.0)	12	22
	No	66(66.0)	18	48	1.118 (0.523-2.387)

^†Adjusted for age, gender, smoking status, alcohol exposure and stage.

* P<0.05.