Figure 4. P72 phenotype promotes p38MAPK and RAF/MEK/ERK signaling.

Molecular analyses revealed that P72^wt or mutant phenotypes effectively induced the activation of p38 MAPK and RAF/MEK/ERK signaling (A and B). Up-regulation of phosphorylated SEK1/MKK4, upstream kinase of p38 MAPK has been found to be associated with P72^wt or mutant phenotypes (A). Activation SEK1/MKK4 was accompanied by up-regulation of p-MAPKAPK-2, p-Hsp27 and p-CREB downstream targets of p38 MAPK (A). Suppression of RAF/MEK/ERK activation was significantly higher in cells that express P72^wt or mutant phenotypes compared to cells that express R72^wt phenotype. This suppression was accompanied by down-regulation of p-Akt (B).