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. 2017 Aug 14;8(44):76949–76960. doi: 10.18632/oncotarget.20270

Figure 2. Culturing and identification of rat NSCs.

Figure 2

(A) Neurospheres in different sizes observed in the culture system at 7 DIV. (B) Most of the cells in the neurosphere are nestin positive NSCs. (C) Some of those cells differentiated into β tubulin III positive neurons in differentiation medium. (D) Some of the cells differentiated into GFAP positive astrocytes in differentiation medium. DIV: days in vitro; DAPI were used for cell nuclei staining and showed in blue. Nestin (B), β tubulin III (C) and GFAP (D) were used for NSCs, neurons and astrocytes identification and showed in green. Scale bar = 50 μm.