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. 2017 Aug 14;8(44):76949–76960. doi: 10.18632/oncotarget.20270

Figure 5. Survival and proliferation of tumor cell in the indirectly co-culture system.

Figure 5

(A) Survival and proliferation of C6 cells in NSC condition medium. (B) Survival and proliferation of U251 cells in NSC condition medium. a. Immunocytochemistry staining showed the ki67 positive proliferating cells. b. The percentage of ki67 positive cells. c. Growth curve of tumor cells. d. Apoptosis and necrosis of tumor cells, detected by FACS. e. Cell cycle distribution of tumor cells, analyzed by FACS. (C) Expression and phosphorylation of AKT and ERK1/2, the level of p53 and caspase 3, detected by Western blot. DAPI used for cell nuclei staining showed in blue, ki67 showed in red. CM means NSC condition medium. Scale bar=50 μm.*p<0.05, **p<0.01 and ***p<0.001.