Figure 7. Invasive ability of tumor cell in the co-cultured system.

(A) Significantly reduced number of C6 cell passed through the transwell membrane, as compared with that cultured in C6 cell medium. (B) Significantly reduced number of U251 cell passed through the transwell membrane, as compared with that cultured in U251 cell medium. Cells underneath the transwell membrane were stained with crystal violet and showed in purple. C6 medium and U251 medium mean their original medium contained DMEM and 10% FBS, NSCs medium means NSC serum free medium. CM means condition medium. Scale bar = 50 μm. *p<0.05.