Figure 7. TUNEL assay and analysis of caspase-3.
Fibroblasts were subjected to PDT (1 mM of MAL plus 11.2 J/cm2 of red light) or UVA/UVB irradiation (500 and 32 mJ/cm2, respectively) plus PDT. (A) TUNEL assay was performed 24 h after treatments.DNA fragmentation (green-stained fragments) typical of apoptotic processes was observed by fluorescence microscopy and the percentages of TUNEL-positive nuclei were measured using the ImageJ software (P<0.01) (scale bar: 20 μm). (B) Post-treatment expression of caspase-3 was quantified by Western blot.Loading control: GADPH (36 kDa).