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. 2017 Aug 24;8(44):77424–77435. doi: 10.18632/oncotarget.20495

Figure 3. GDF7 promoted tenogenic differentiation of hMSCs.

Figure 3

(A) Evaluate the expression levels of GDF6 and GDF7 in GFP-positive and negative hMSCs by quantitative RT-PCR. β-actin was used as an internal control. The data are expressed as mean ± SD (n=3), *p<0.05. (B) GDF7 at different dosage was supplemented in the tenogenic differentiation medium for 7 days, then total RNA was extracted to evaluate the relative expression levels of ACAN, Scx, TNC, Tnmd, Fmod and Collagen type I. β-actin was used as an internal control. The data are expressed as mean ± SD (n=3), *p<0.05. (C) GDF7 at different dosage was supplemented in the tenogenic differentiation medium for 7 days, then immunocytochemistry staining was performed to observe the expression of Tena C, Tnmd, and Collagen type I.