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. 2017 Sep 5;8(44):77552–77566. doi: 10.18632/oncotarget.20641

Figure 4. TP15-Fc specifically binds human, but not mouse ICAM-1.

Figure 4

(A) Flow cytometric analyses of non-transfected and stably transfected CHO-K1 cells with varying ICAM-1 expression levels detected by CD54-FITC (Beckman Coulter; dark grey) correlated with binding of 10 μg/ml TP15-Fc (light grey). 4D5-Fc was used as control (black). (B) ICAM-1 expression of non-transfected (upper row, left) and human ICAM-1 transfected Lenti-X 293T cells (upper row, middle) was measured by flow cytometry using mouse anti-human CD54-FITC (grey) and a FITC-labeled isotype control (black). Mouse ICAM-1 was expressed as GFP-tagged protein and positive cells were detected by fluorescence (upper row, right; grey histogram). Non-transfected cells served as control (black). Binding of 100 μg/ml TP15-Fc (grey) and 4D5-Fc (black), respectively, on non-transfected and ICAM-1-positive cell populations was detected with a PE-labeled anti-human Fcγ-specific secondary antibody (lower panel).