Figure 2. H₂O₂ induced autophagy of resident CSCs.

(A) CSCs were treated with indicated concentrations of H₂O₂ for 5 h, changes of LC3-II were observed by western blot. (B and C) CSCs were pretreated with 3MA (5 mM) or Baf A1 (50 nM) for 30 min followed by H₂O₂ treatment for 5 h. The levels of LC3-II and P62 were detected by western blot. ctr, control. *P < 0.05; **P < 0.01; n=3.