Figure 5. NR4A2 participated in H₂O₂-induced autophagy and apoptosis in CSCs.

CSCs were treated with LV5-NR4A2 for 48 h. (A) The fluorescent images showed the transfection efficiency. (B and C) NR4A2 changes in mRNA and protein were detected by qPCR (B) and western blot (C). (D) cleavage of caspase 3 and PARP1, LC3-II and P62 were detected by western blot. (E) CSCs with NR4A2 knockdown were transfected with NR4A2 overexpression plasmid or the negative control, then NR4A2 changes in mRNA and protein were detected by qPCR. (F) cleavage of caspase 3 and PARP1, and LC3-II and P62 in H₂O₂-treated CSCs were detected by western blot. *P < 0.05; **P < 0.01; n=3.