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. 2017 Sep 8;8(44):77685–77695. doi: 10.18632/oncotarget.20768

Figure 1. Cigarette smoke (CS) induces endoplasmic reticulum (ER) stress.

Figure 1

(A) Beas-2B cells were treated with 2% CSE for indicated time. (B) Beas-2B cells were treated with indicated concentrations of CSE for 12h. The CSE-induced ER stress was assessed by western blot analyses for IRE1α, GRP78 and CHOP. Western blot analyses represent of 3 independent experiments (A and B). Wild type mice were exposed to air or CS for 3months, and mouse lung homogenate samples were analyzed for the indication of ER stress. (C and D) Western blot analyses and densitometric analyses showed enhanced levels of IRE1α, GRP78 and CHOP in CS-exposed mice lungs (lanes 7-12), compared with air exposure (lanes 1-6). Quantification of band intensities were normalized to actin. Bars represent means± SEM. *P < 0.05, **P < 0.01 (Student's t-test). All results represent of 3 independent experiments.