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. 2017 Sep 8;8(44):77685–77695. doi: 10.18632/oncotarget.20768

Figure 5. Effect of 4-PBA on CS-induced emphysema and alveolar cells apoptosis in mouse lungs.

Figure 5

Mice were administered with PBS or 4-PBA and then exposed to room air or CS for 6 months. (A) Representative micrographs (n = 10 images per mouse lung; n = 5 mice per group) of mouse lungs with H&E staining and mean linear intercept of mice lungs were measured. Scale bars: 100 μm (insets: 50 μm). (B) Representative images (n = 8-10 images per mouse lung; n = 5 mice per group) of immunohistochemistry staining for cleaved caspase-3 in lung section (left). Cleaved caspase-3-positive alveolar septal cells were counted and alveolar cells positive for the Cleaved caspase-3 antibody staining were expressed as a percentage of total cells (right). Scale bars: 50 μm. (C) Representative images (n = 8-10 images per mouse lung; n = 5 mice per group) of TUNEL staining in lung section (left). TUNEL-positive alveolar septal cells were counted and alveolar cells positive for the TUNEL staining were expressed as a percentage of total cells (right). Scale bars: 50 μm. Results were shown at means± SEM. *P < 0.05, **P < 0.01, (Student's t-test).