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. 2017 Sep 12;8(44):77860–77871. doi: 10.18632/oncotarget.20842

Figure 1. The mRNA and protein expressions of carbonic anhydrase IX (CAIX) in different cancer cell lines.

Figure 1

(A) The mRNA and (B) protein expressions of CAIX in SiHa cervical cell lines were lowest among SiHa, HeLa and Caski cancer cell lines of uterine cervix. The (C) mRNA and (D) protein expressions of CAIX were elevated in SiHa cell lines with CAIX gene transfection. The mRNA and protein expressions of CAIX were detected by RT-PCR and Western blot analysis. GAPDH and β-actin were separately used as internal controls. The (E) migratory and (F) invasive abilities of pcDNA3.0 and pcDNA3.0-CAIX SiHa cells were evaluated using Boyden chamber migration and Matrigel invasion assays. Differences are presented as the mean of triplicate experiments compared with control cells. *p < 0.05 compared with control cells.