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. 2017 Sep 12;8(44):77860–77871. doi: 10.18632/oncotarget.20842

Figure 2. Effect of miR-34a inhibitor and mimic on the protein expressions of carbonic anhydrase IX (CAIX), migratory and invasive abilities in different cancer cell lines.

Figure 2

Reduced expressions of miR-34a after miR-34a inhibitor transfection into (A) HeLa and (B) SiHa cancer cell lines of uterine cervix. Elevated expressions of miR-34a after miR-34a mimic transfection into (C) HeLa and (D) SiHa cancer cell lines. The RNA levels were determined by Real time PCR 24 hours after miR-34a mimics and inhibitors transfection into these cancer cells. (E) Elevated expressions of CAIX protein after 200 nM miR-34a inhibitor transfection into HeLa and SiHa cancer cell lines of uterine cervix. (F) Reduced expressions of CAIX protein after 100 nM miR-34a mimic transfection into HeLa and SiHa cancer cell lines. The protein levels were determined by Western blot 24 hours after miR-34a inhibitors and mimics transfection into these cancer cells. (G-H) Increased migratory abilities in (G) HeLa and (H) SiHa cervical cancer cells with miR-34a inhibitor, as compared to their negative control cells without inhibitor by Boyden chamber assay.