Stage IV							Stage III
		CTCs (n)			McNemar-Test	Kappa	P value		CTCs (n)			McNemar-Test	Kappa	P value
		Mutation Codon 12+13	Wild type	Total					Mutation Codon 12+13	Wild type	Total
Tissue sample (n)	Mutation Codon 12+13	35	39	74	< 0.001*	0.377	< 0.001*	Mutation Codon 12+13	19	2	21	0.687	0.744	< 0.001*
	Wild type	10	81	91				Wild type	4	22	26
	Total	45	120	165				Total	23	24	47
		Mutation Codon12	Wild type	Total	McNemar-Test	Kappa	P value		Mutation Codon12	Wild type	Total	McNemar-Test	Kappa	P value
Tissue sample (n)	Mutation Codon12	30	34	64	< 0.001*	0.397	< 0.001*	Mutation Codon12	19	2	21	0.500	0.913	< 0.001*
	Wild type	10	91	101				Wild type	0	26	26
	Total	40	125	165				Total	19	28	47
		Mutation Codon13	Wild type	Total	McNemar-Test	Kappa	P value		Mutation Codon13	Wild type	Total	McNemar-Test	Kappa	P value
Tissue sample (n)	Mutation Codon13	4	6	10	0.289	0.476	< 0.001*	Mutation Codon13	0	0	0	NA	NA	NA
	Wild type	2	153	155				Wild type	4	43	47
	Total	6	159	165				Total	4	43	47

*:indicate P <0.05, NA: not available.