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. 2017 Jul 28;38(4):1932–1940. doi: 10.3892/or.2017.5855

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Copyright: © Doan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — U87-10gy and SJGBM2-10gy cells are sensitive to carmofur and inhibition of ASAH1 with carmofur leads to increased levels of ASAH1 substrate ceramides. (A) Cells treated with carmofur and their IC₅₀ values of 37, 50, 28 and 21 µM for U87, SJGBM2, U87-10gy and SJGBM2-10gy, respectively, were determined utilizing MTT assays. (B) Intracellular ceramide levels were measured from untreated U87-10gy cells and U87-10gy cells treated with 50 µM carmofur for 1 h. Concentrations were normalized and adjusted based on the phosphate level. C(N), ceramide species with long-N-acyl-chain; dhC, dihydroceramides. Results shown are mean ± SD of three independent experiments. *P<0.05.