Figure 4.
In vitro efficiency of the siRNA batches used in vivo. (A) Luciferase-targeted siRNAs strongly decrease target expression in vitro. Transfection was performed with siLuc in SKOV3-Luc cells with final 20 nM of siRNAs, and target downregulation analyzed 72 h after transfection. Bioluminescent activity was measured in cell lysates. Results are relative to protein concentration in respective cell lysates ± SD. (B) Both Bcl-xL- and Mcl-1-targeted siRNAs mediate strong downregulation in vitro on their respective targets. Transfection was performed with siGFP, siMcl1 or siBcl-xL in SKOV3 cells with final 20 nM of siRNAs, and target downregulation analyzed 72 h after transfection. Mcl-1 and Bcl-xL protein expression was assessed by western blotting. Actin was used as a loading control.