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. 2017 Aug 3;38(4):1959–1966. doi: 10.3892/or.2017.5878

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effects of juglone on Pin1, TGF-β1, Smad2/3 and miR-21 expression. U251 cells were treated with 5, 10 and 20 µM juglone for 24 h. (A) Western blot analysis of Pin1 in HUVECs with the indicated treatments. (B and C) Juglone-induced downregulation of TGF-β mRNA and protein expression, as determined by qPCR and western blotting. (D) Juglone promoted a decrease in phospho-Smad2/3, as determined by western blotting. (E) Bar diagram summarizing the effects of juglone on miR-21 expression by qPCR. Data are shown as mean ± SD of three independent experiments, *P<0.05 vs. control.