Table III.
Functional evaluation of amino acid substitutions using bioinformatics tools.
| Type of mutations | TP53 | TP63 | TP73 | TP53 vs. TP63 (P-value) |
|---|---|---|---|---|
| No. of mutations | 105 | 111 | 10 | |
| Single bps | 88 | 101 | 10 | |
| Missense mutations with predicted functional consequences according to: | ||||
| Mutation Assessor | 64 | 95 | 9 | |
| Neutral | 2 | 42 | 6 | <0.0001 |
| Low | 2 | 21 | 1 | <0.009 |
| Medium | 60 | 32 | 2 | <0.0001 |
| High | 0 | 0 | 0 | |
| FATHMM prediction | 88 | 79 | 7 | |
| Neutral | 2 | 0 | 3 | NS |
| Pathogenic | 86 | 79 | 4 | NS |
Since the numerosity of the TP73 group is one order of magnitude lower than those of the TP63 and TP53 samples, only statistical comparisons performed between the TP53 vs. TP63 group of samples are reported. NS, not significant.