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. 2017 Aug 8;38(4):2116–2122. doi: 10.3892/or.2017.5890

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Copyright: © Cheng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Knockdown of BZW2 inhibits the Akt/mTOR signaling pathway in osteosarcoma cells. (A and D) MNNG/HOS and U2OS cell extracts were prepared and analyzed using the PathScan Intracellular Signaling Antibody Array kit (#7744). Images were captured using the Odyssey^® Infrared Imaging System (LI-COR). (B and E) Diagrams show the quantitative results for MNNG/HOS and U2OS cells. (C and F) Western blot analysis of the Akt/mTOR signaling pathway, including mTOR and Akt, in MNNG/HOS and U2OS cells transfected with si-NC or si-BZW2.