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. 2017 Oct 1;7(8):526–540. doi: 10.1089/brain.2017.0486

FIG. 1.

FIG. 1.

Quantitative mapping of functional network alterations in Gpr88−/− mice reveals a strong Gpr88-dependent activity signature in live animals: (a) Group-specific resting-state FC matrices (CTRL above and Gpr88−/− group below the diagonal), including both positive and negative internodal correlations (derived via partial correlation methods). The nodes were defined through functional segregation using 100-ICASSO. Functional nodes were grouped and assigned to corresponding anatomical regions through coregistration on the Allen Mouse Brain Atlas (d). (b) Direct intergroup (CTRL vs. Gpr88−/−) statistical comparison of connectivity matrices (two-sample t-test, p < 0.01, FDR corrected) is shown as a 2D matrix. The Gpr88 genetic inactivation induced widespread modifications of internode connectivity. The red circle points to the cluster of strong intracortical FC remodeling in the absence of Gpr88, whereas the red square points to extensive significant FC alterations of the RSP area connectivity. (c) Nodes of brain area with highest number of statistically significant connectivity changes are ranked (two-sample t-test, p < 0.01, FDR corrected). Their functional pattern is overlaid on four T2-weighted anatomical images. From top to bottom: retrosplenial area (RSP), somatosensory area (SS), motor area (MO), visual area (VIS), thalamus (TH), temporal association area (TEa), superior colliculus (SC), caudate-putamen (CP), agranular insular area (AI), hippocampal formation (HPF), and anterior cingulate area (ACA). (d) Assignment of brain regions from rostral to caudal direction and their corresponding localization on the sagittal mouse brain template adapted from the Allen Mouse Brain Atlas. The anatomical overlap of components was identified using a MATLAB-based and in-house-developed postprocessing tool, which is based on coregistration of the data on the Allen Mouse Brain Atlas (AMBA) (Lein et al., 2007) (http://mouse.brain-map.org/static/atlas). We assigned the component to the region or anatomical subdivision (in case of large areas) with maximum overlay. Nevertheless, components are partly touching other regions (subdivisions). CTRL, control; FC, functional connectivity; FDR, false discovery rate; GPR88, G protein-coupled receptor 88; RSP, retrosplenial.