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Intrinsic tryptophan fluorescence shift assay. Peak wavelengths for glucagon samples tested at different physical conditions. M is for movement and B is for air bubbles. Samples are incubated in infusion pump sets for 24 h at 32°C immediately postreconstitution for S1–S4, 24 h postincubation at 4°C for S5–S8, and for 48 h postincubation at 4°C for S9–S12. Aged glucagon is kept for 30 days at 4°C, n = 3.
