Fig. 4.

Effects of surface modifications on tumor-specific targeting of dendrimer conjugates. KB cells were incubated with 10 nM of the targeted, surface-modified dendrimer conjugates for 2 h. After incubation, the cells were harvested and AF647 was conjugated in situ using the CuAAC approach. Dendrimer uptake was then measured using flow cytometry. Surprisingly, the acetylated targeted dendrimer conjugate (6) demonstrated significantly more uptake than both the PEGylated targeted dendrimer conjugates (7 and 8). ^*P < 0.05 for NT and treatments (One way Anova, Dunnett’s post hoc test). ^**P < 0.05 between G5-Alkyne-FA-NHAc (6) and other treatments (One way Anova, Dunnett’s post hoc test). Results are representative of 3 independent experiments.