Fig. 4.

Induction of chemokine gene expression by lipopolysaccharide. Ferret peripheral blood was stimulated with 0.1 mg/mL LPS for the time points indicated. Utilizing primers specific for the ferret CXCL9, CXCL10, and CXCL11 genes, mRNA expression was quantified. The Y-axis indicates the abundance of mRNA after stimulation with LPS compared to untreated samples. Treatment time is indicated by X-axis. Error bars indicate the S.D. of the relative quantities of the triplicates. Quantity readings of triplicates at each time point are compared with quantity readings of unstimulated triplicates, using Student's t-test. The peak increases for CXCL9 and CXCL11 occurred at 4 h post-stimulation (P < 0.05 and P < 0.01, respectively), while for CXCL10 was at 2 h following LPS treatment (P < 0.01) (*P < 0.05, **P < 0.01).