Fig. 4.
Activity column profiles from Ni2+-NTA/FPLC (A) or from heparin column/FPLC system (B). Fractions were assayed for strand-transfer activity (upper panels) and SDS-PAGE and Coomassie staining (lower panels). (C) M-MuLV IN proteins purified using batch elution. Lane 1, IN 1–105; lane 2, IN 106–404; lane 3, C209A IN; lane 4, WT IN.