Skip to main content
. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Birth Defects Res. 2017 Jul 6;109(15):1212–1227. doi: 10.1002/bdr2.1069

Fig. 8. Ethanol had a minimal effect on the expression of periocular and ocular genes associated with congenital eye diseases.

Fig. 8

Semi-quantitative RT-PCR showed that 1% or 3% ETOH treatment from 24 to 48 hpf had minimal effect on expression of pitx2a, and foxc1a in RNA isolated from whole 48 hpf embryos. Foxc1b expression was decreased after treatment with 3% ETOH, but not 1% ETOH. Whole mount in situ hybridization in untreated (B), 1% ETOH treated (B′), and 3% ETOH treated (B″) 48 hpf embryos demonstrated that pitx2a was expressed in the pituitary (yellow arrows), periocular mesenchyme, and the ocular fissure (open arrows). Foxc1a was expressed in the facial mesenchyme, periocular mesenchyme (yellow arrows), and in the ocular fissure (open arrows) of untreated (C), 1% ETOH treated (C′), and 3% ETOH treated (C″) 48 hpf embryos. Similar to foxc1a, foxc1b was expressed in the facial mesenchyme (black arrows) and periocular mesenchyme (open arrows), but not in the ocular fissure of untreated (D) and 1% ETOH treated (D′) 48 hpf embryos. Treatment with 3% ETOH decreased foxc1b expression (D″) in the facial mesenchyme and periocular mesenchyme.