Table 1. Mass isotopomer distribution of the TCA cycle intermediates.
Metabolite | M+0 (SD) | M+1 (SD) | M+2 (SD) | M+3 (SD) | M+4 (SD) |
---|---|---|---|---|---|
Citrate | 33% ±1.53 | 17% ±1.56 | 24% ±1.45 | 18% ±1.1 | 8% ±1.13 |
2-Oxoglutarate | 47% ±0.15 | 12% ±0.34 | 25% ±1.23 | 11% ±0.22 | 6% ±1.99 |
Succinyl-CoA | 50% ±2.08 | 14% ±1.68 | 19% ±3.08 | 12% ±2.73 | 5% ±1.69 |
Succinate | 50% ±0.09 | 15% ±0.14 | 10% ±1.21 | 21% ±0.22 | 4% ±1.09 |
Fumarate | 47% ±0.15 | 20% ±0.48 | 16% ±0.18 | 17% ±0.74 | |
Malate | 45% ±0.05 | 21% ±0.43 | 15% ±0.02 | 17% ±0.33 |
For GC/MS data n = 3, for HPLC/MS data (Succinyl-CoA) n = 4. Not determined mass isotopologue fractions are not displayed. SD = relative standard deviation.