Table 3. Effective Mating, Relatedness, Sperm Count and Paternity Skew in the control and neonicotinoid treated honeybee (Apis mellifera) queens.

	Colony	Colony source	N	Observed Effective Mating	Observed Relatedness	Corrected Effective Mating	Corrected Relatedness	Sperm Count (x106)	Paternity skew
Control	1	1C	24	11.62	0.29	19.71	0.27	4.78	0.35
	2	1C	24	8.73	0.31	13.14	0.29	2.85	0.22
	3	1C	24	11.52	0.29	21.23	0.27	5.78	0.32
	4	1C	24	14.40	0.28	34.50	0.26	5.65	0.33
	5	1C	24	10.67	0.30	18.40	0.28	3.13	0.38
	6	3C	24	13.40	0.29	29.13	0.27	2.32	0.38
	7	3C	20	10.53	0.30	21.11	0.27	6.45	0.36
	8	3C	24	12.00	0.29	23.00	0.27	5.75	0.35
	9	3C	22	6.72	0.32	9.24	0.30	6.50	0.35
	10	5C	24	14.40	0.28	34.50	0.26	5.55	0.33
	11	5C	22	15.13	0.28	46.20	0.26	4.95	0.32
	12	5C	22	11.52	0.29	23.10	0.27	4.32	0.34
			Mean	11.72	0.29	24.44	0.27	4.84	0.34
			± SD	± 2.44	± 0.01	± 10.18	± 0.01	± 1.40	± 0.01
Neonicotinoid	1	2P	24	8.73	0.31	13.14	0.29	2.95	0.32
	2	2P	24	9.93	0.30	16.23	0.28	4.77	0.36
	3	2P	24	10.67	0.30	18.40	0.28	4.44	0.31
	4	4P	24	13.09	0.29	27.60	0.27	0.87	0.22
	5	4P	22	8.22	0.31	12.57	0.29	5.30	0.37
	6	4P	23	9.28	0.30	14.88	0.28	2.37	0.27
	7	4P	24	10.29	0.30	17.25	0.28	3.75	0.34
	8	4P	20	5.26	0.34	6.79	0.32	4.97	0.33
	9	6P	23	10.8	0.30	19.46	0.28	4.07	0.28
	10	6P	24	8.00	0.31	11.50	0.29	7.25	0.38
	11	6P	24	7.58	0.32	10.61	0.30	3.00	0.48
	12	6P	24	8.47	0.31	12.54	0.29	5.65	0.56
			Mean	9.19	0.31	15.08	0.29	4.11	0.35
			± SD	± 1.97	± 0.01	± 5.32	± 0.01	± 1.68	± 0.03

Observed Effective Mating = number of male mates if all are equally represented in the queen’s offspring; Corrected Effective Mating = Observed Effective Mating corrected for sampling size; Observed Relatedness = intracolonial genetic relatedness; Corrected Relatedness = intracolonial genetic relatedness corrected for sampling size.