Table 2. Levels of inhibition of the mRNA and protein expression of viral genes in cells expressing ribozymes, as compared to the levels of inhibition in cells that did not express a ribozyme (U251).
Viral gene | Gene class | U251 | C-M1-IE | C-R228-IE | F-M1-IE | F-R228-IE | F-M1-TK |
---|---|---|---|---|---|---|---|
IE1 mRNA | IE | 0% | 6% | 8% | 77±7% | 99±9% | 0% |
IE2 mRNA | IE | 0% | 5% | 7% | 79±7% | 98±8% | 0% |
US2 mRNA | Early | 0% | 2% | 2% | 78±6% | 98±9% | 0% |
IE1 protein | IE | 0% | 5% | 7% | 77±7% | 99±8% | 0% |
IE2 protein | IE | 0% | 5% | 6% | 78±8% | 98±9% | 0% |
UL99 protein | Late | 0% | 0% | 0% | 79±7% | 98±8% | 0% |