Table 1. The Top30 most upregulated genes in ΔZtGT2 vs WT fungus growing in liquid medium.
*Gene_id new Ensembl | **Original Id Ensembl | FPKM WT | FPKM ΔZtGT2 | Log2 fold change ΔZtGT2/WT | Blastp annotation (NCBI) | Signal peptide? | Transmembrane? | log2 fold WT 48h plant / culture |
---|---|---|---|---|---|---|---|---|
ZtritIPO323_04t13800 | Mycgr3G47776 | 2.38361 | 187.623 | 6.29855 | GPR1 FUN34 -class plasma membrane | No | YES | 3.69719 |
ZtritIPO323_04t06606 | Mycgr3G103427 | 36.5046 | 2328.37 | 5.9951 | Tandem Repeat Protein 4 (TRP4)*** | Yes | No | 4.56836 |
ZtritIPO323_04t08955 | Mycgr3G109137 | 50.7599 | 2372.23 | 5.54641 | hypothetical protein MYCGRDRAFT_109137 | Yes | No | 4.48632 |
ZtritIPO323_04t03143 | Mycgr3G111221 | 21.2326 | 742.174 | 5.12741 | 3xLysM domain effector (3LysM) | Yes | No | 5.30617 |
ZtritIPO323_04t10723 | Mycgr3G104794 | 327.83 | 10093.8 | 4.94438 | hypothetical protein MYCGRDRAFT_104794 | Yes | No | 1.90893 |
ZtritIPO323_04t01868 | Mycgr3G89984 | 9.07987 | 277.305 | 4.93266 | hypothetical protein MYCGRDRAFT_89984 | No | YES | 2.02453 |
ZtritIPO323_04t07933 | Mycgr3G91855 | 5.51628 | 168.164 | 4.93003 | aspergillopepsin-2 precursor like | Yes | No | No change |
ZtritIPO323_04t01956 | Mycgr3G67250 | 4.68437 | 124.825 | 4.73591 | peroxidase catalase like | Yes | No | 1.71448 |
ZtritIPO323_04t00231 | Mycgr3G34306 | 5.01835 | 132.068 | 4.71792 | Aspartic protease pep1 like | Yes | No | 3.47866 |
ZtritIPO323_04t13637 | Mycgr3G95831 | 20.0937 | 519.83 | 4.69323 | hypothetical protein MYCGRDRAFT_95831 | Yes | No | No change |
ZtritIPO323_04t04294 | Mycgr3G111636 | 235.826 | 5976.16 | 4.66342 | Ecp2-3 effector homologue | Yes | No | 1.9105 |
ZtritIPO323_04t08501 | Mycgr3G104000 | 38.9405 | 950.309 | 4.60905 | hypothetical protein MYCGRDRAFT_104000 | Yes | No | 6.39787 |
ZtritIPO323_04t10384 | Mycgr3G72646 | 6.27375 | 148.704 | 4.56697 | alpha 1,3 glucan synthase | No | YES | 1.76247 |
ZtritIPO323_04t11777 | Mycgr3G110220 | 32.942 | 745.202 | 4.49963 | hypothetical protein MYCGRDRAFT_110220 | Yes | No | 2.4987 |
ZtritIPO323_04t04149 | Mycgr3G77689 | 2.1705 | 43.3503 | 4.31994 | carboxypeptidase S1 | Yes | No | 5.99527 |
ZtritIPO323_04t03806 | Mycgr3G97031 | 19.0535 | 376.75 | 4.30548 | hypothetical protein MYCGRDRAFT_97031 | Yes | No | No change |
ZtritIPO323_04t09707 | none | 28.0532 | 550.981 | 4.29576 | none | Yes | No | No change |
ZtritIPO323_04t13185 | Mycgr3G48129 | 91.2925 | 1728.88 | 4.2432 | Cerato-platanin / hydrophobin | Yes | No | No change |
ZtritIPO323_04t11297 | Mycgr3G12051 | 1.65444 | 28.5554 | 4.10935 | galactose oxidase | Yes | No | 4.3809 |
ZtritIPO323_04t09426 | Mycgr3G71659 | 4.53293 | 77.3318 | 4.09255 | NAD(P)-binding Rossmann-fold containing | No | No | No change |
ZtritIPO323_04t07171 | Mycgr3G108399 | 38.5037 | 644.32 | 4.06471 | hypothetical protein MYCGRDRAFT_108399 | No | No | No change |
ZtritIPO323_04t03262 | Mycgr3G96651 | 1.87684 | 31.0977 | 4.05043 | hypothetical protein MYCGRDRAFT_96651 | No | No | No change |
ZtritIPO323_04t04165 | none | 9.77584 | 156.07 | 3.99683 | hypothetical protein TI39_contig4278g00011 | Yes | No | 5.34383 |
ZtritIPO323_04t00584 | none | 77.6554 | 1221.01 | 3.97484 | hypothetical protein TI39_contig279g00027 | No | No | No change |
ZtritIPO323_04t09555 | Mycgr3G92998 | 25.6961 | 402.709 | 3.97012 | hypothetical protein MYCGRDRAFT_92998 | Yes | No | 1.87831 |
ZtritIPO323_04t06766 | Mycgr3G69117 | 4.18014 | 64.4432 | 3.9464 | MFS monosaccharide transporter like | No | YES | No change |
ZtritIPO323_04t11928 | Mycgr3G19274 | 257.455 | 3908.59 | 3.92425 | major allergen alt a1 | Yes | No | No change |
ZtritIPO323_04t13136 | Mycgr3G46937 | 8.05712 | 121.448 | 3.91393 | major facilitator superfamily transporter like | No | YES | No change |
ZtritIPO323_04t12624 | Mycgr3G105419 | 276.522 | 4164.69 | 3.91274 | small threonine-rich | Yes | No | -1.58721 |
ZtritIPO323_04t13161 | Mycgr3G101121 | 2.95145 | 42.827 | 3.85902 | general substrate transporter | No | YES | 5.83776 |
*- sequences available from https://doi.org/10.6084/m9.figshare.4753708.v1 [42].
** sequences available from http://fungi.ensembl.org/Zymoseptoria_tritici/Info/Index.
*** previously described in [43].