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. 2017 Oct 23;12(10):e0186517. doi: 10.1371/journal.pone.0186517

Fig 6. Effect of ROS modulation on sirtuin activity.

Fig 6

(A) Relative amount of reactive oxygen species (ROS) in COX-deficient cells compared to control cells (B) Detection of relative amount of ROS in control cells treated with ROS inducers (paraquat, diclophenac) or ROS scavenger (tocopherol) (C-D) Enzymatic activities of SIRT1 (C) and SIRT 3 (D) in control cells treated with ROS inducers (paraquat, diclophenac) or ROS scavenger (tocopherol) (E-F) Relative transcript levels of SIRT1 (E) and SIRT 3 (F) after treatment with a ROS scavenger (tocopherol) or ROS inducers (paraquat, diclophenac) in control fibroblasts (G-H) Relative protein amount of SIRT1 (G) and SIRT 3 (H) in control cells treated with ROS inducers (paraquat, diclophenac) or ROS scavenger (tocopherol); (n = 3; all data are shown in mean ± SD; * p < 0.05).