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HDX back exchange as a function of the incubation time (e.g., 3 and 48 h) and the time after desolvation (e.g., 0, 100, and 500 ms), in terms of charge state and CCS in the positive ion mode. Clustering of the number of kinetically trapped intermediates and the initial levels of exchange allows the identification of four groups with different levels of exchange protection (color rectangles and data points).
