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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Virology. 2017 Dec;512:95–103. doi: 10.1016/j.virol.2017.09.009

Figure 7. UL97 directs FoxM1 phosphorylation on Thr600.

Figure 7

(A) Saos-2 cells were transfected with expression plasmids for T7-tagged FoxM1 together with either an empty vector (EV), HA-tagged wild-type UL97 (97), the indicated allele of UL97, or cyclin E1/CDK2. Lysates harvested 48 hr after transfection were analyzed by western blot with the indicated antibodies. (B) NHDFs were transfected with the CMV promoter-driven T7-tagged FoxM1. At 18 hr post-transfection the transfected NHDFs were serum-starved. After 48 hr cells were infected with HCMV at an MOI of 3. At 2 hr post-infection infected cells were treated with 10 μM maribavir (MBV) or DMSO as a vehicle. Whole cell lysates were prepared at 48 hr post-infection and analyzed by western blot with the indicated antibodies.