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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Mol Microbiol. 2017 Aug 29;106(3):335–350. doi: 10.1111/mmi.13768

Fig. 1. Effects of HOCl, HOBr and HOSCN on the survival of non-growing or exponentially growing PA14.

Fig. 1

Wild-type PA14 was grown in MOPS-glucose media to mid-log phase (A600= 0.4–0.5) and shifted to either (A) PBS buffer or (B) MOPS media in the absence or presence of glucose. The indicated concentrations of HOCl, HOBr, or HOSCN were added. After 30 min incubation, excess HOX was quenched by the addition of 10 mM thiosulfate, and the cells were serially diluted with 0.85% NaCl, spot-titered onto LB agar plates, and incubated o/n at 37°C. The experiments were repeated at least three independent times. Data shown in (A) represent means ± s.e.m. A representative data set is shown in (B).